What is Blood Transcriptomics? Considering that disease-causing and disease-protecting immune cells need to be transferred from lymphatic and secondary lymphoid organs through the peripheral circulation to their sites of action in disease, a clearer picture of the cells and their action gene expression is provided by identifying the components in whole blood. Blood transcriptome sequencing...

Overview The breakthrough of extracellular RNAs (exRNAs) in biofluids has sparked a lot of curiosity about their biological functions and clinical applications. ExRNA biomarkers have been revealed as diagnostic, prognostic, and theranostic for a variety of diseases. Few have been verified across studies, most likely due to methodological differences between studies, such as exRNA isolation and...

Introduction to Epigenetic Profiling Epigenetic profiling is essential for elucidating gene regulation and chromatin dynamics. Chromatin Immunoprecipitation sequencing (ChIP-seq) has long been the gold standard for mapping protein-DNA interactions and histone modifications. However, the recent advent of Cleavage Under Targets and Release Using Nuclease (Cut&Run) has...

Overview of DAP-seq The DNA Affinity Purification Sequencing (DAP-seq) technique is an innovative method aimed at exploring the interactions between transcription factors (TFs) and genomic DNA. By simulating the interaction between TFs and their DNA binding sites in vitro, this technology reveals key DNA sequences in gene regulatory networks. In contrast to traditional Chromatin...

What is Microbial Sulfur Cycling? Sulfur, an indispensable nutrient for all life forms, serves as a vital building block for amino acids such as methionine and cysteine. Abundant sulfur reservoirs exist in nature, particularly in the marine ecosystem, where sulfate and sulfide are stored in copious amounts. The transformation of sulfur compounds with varying valence states primarily relies on...

Viral metagenomics is the study of viruses in environmental and biological samples by utilizing next generation sequencing that generates very large data sets. Viral metagenomics analyzes viral sequences to deduce the impact of viruses on the environment of human health. Unlike amplicon sequencing, metagenomics obtains and investigates genetic material directly from...

In recent years, the continuous advancements in sequencing technology have markedly refined genome sequencing techniques, yielding significant achievements in both animal and plant genomic research. Numerous plant genome drafts and detailed maps have emerged, supplying invaluable resources for the scientific community. This article provides an in-depth analysis of the characteristics...

The bovine genome, comprising the entire genetic blueprint of cattle, has been a subject of extensive research and exploration. In recent years, breakthroughs in genomics have enabled scientists to delve deep into the genetic makeup of bovines, leading to remarkable advancements in understanding bovine traits, parentage verification, and harnessing genetic potential for improved breeding...